1.
Microsc. electron. biol. celular
;
14(2): 139-46, 1990.
Article
in English
| LILACS
| ID: lil-121627
ABSTRACT
A pigeon crop-sac bioassay for prolactin was developed applying a morphometric approach for quantification of the epithelial cell proliferation induced by the hormone. A small male dove, indigenous from South America (Zenaida auriculata) previously castrated was used as experimental animal. The total dose of prolactin was divided into 4 daily injections administered systemically into the pectoral muscles. The number of the proliferated cell layers ysed as the bioassay end point was analyzedstatistically. This procedure yields a highly sensitive bioassay with an excellent precision index, particularly well suited for quantification of the different molecular forms of pituitary PRL
Subject(s)
Animals , Male , Biological Assay , Columbidae , Crop, Avian/drug effects , Prolactin/analysis , Crop, Avian/ultrastructure , Cell Division , Dose-Response Relationship, Drug , Epithelium/drug effects , Epithelium/ultrastructure , Injections, Intramuscular , Prolactin/administration & dosage , Prolactin/pharmacology , Stimulation, Chemical
2.
Indian J Exp Biol
;
1988 Oct; 26(10): 768-70
Article
in English
| IMSEAR
| ID: sea-57704
Subject(s)
Animals , Chickens , Crop, Avian/drug effects , Esophagus/drug effects , Female , Histamine/toxicity , Male , Promethazine/toxicity , Proventriculus/drug effects
3.
Indian J Exp Biol
;
1971 Apr; 9(2): 260-1
Article
in English
| IMSEAR
| ID: sea-60935